National Core Facility for Biopharmaceuticals

TRIM

Management and Value-Added Applications of the Taiwan Rice Mutant Library and DatabaseHo, Tuan-Hua

Rice is one of the most important staple food crops in the world. Taking advantage ofthe knowledge and experience of rice functional genome research, we have combined modern and traditional technologies, to improve and breed new varieties of rice and other cereal crops for enhancing resistance and maintaining grain yield under adverse environment due to global climate changes.
Almost 20 years ago, our research team has generated a rice mutant (Taiwan Rice Insertional Mutant Database, TRIM) library following a T-DNA insertional mutagenesis approach. Approximately 100,000 gene knockout or activation mutant lines have been generated, and 60,000 flanking sequences of T-DNA insertion sites in the rice genome have been determined. A database containing flanking sequences and mutant phenotypes has been established and is made available to the public via a well-designed website. These resources are extremely useful for rice functional genomics research following either forward or reverse genetics approaches. The TRIM library and database have been well-recognized and gained recognition in the international plant science community. Furthermore, it is one of the only two (Taiwan and Korea) gene activation mutant libraries available to the public in the world. Currently, seed orders come from all over the world and dozens of novel genes have been identified due to the use of TRIM library, and we are also in the process of cooperating with several international academic institutions, and have generated a number of important publications and patents.

https://rice.sinica.edu.tw/

Acknowledgment template：

We thank the Taiwan Rice Insertional Mutant (TRIM) database Core Facility (TRIM Core) of the National Core Facility for Biopharmaceuticals at Academia Sinica in Taiwan for providing T-DNA insertional mutants and related services.